Difference between revisions of "Part:BBa K1033933:Experience"

(→‎Applications of BBa_K1033933)
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2019 iGEM team Linkoping Sweden validated this part.<br><br>
 
2019 iGEM team Linkoping Sweden validated this part.<br><br>
  
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To test the bindning capacity of CBD-asPink (<partinfo>BBa_K3182000</partinfo>) the white microbial cellulose bandage was into the sonicated lysate of BL21(DE3) with the expressed fusion protein (see <b><I>Figure 1</I></b>) and incubated for 30 minutes.
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The bandage was then washed thrice in 70% ethanol which confirmed that the bindning of CDB-asPink was still intact after the washes to the bandage.
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[[File:T--Linkoping Sweden--aspink bunden.jpeg|150px|left|thumb|<b><I>Figure 1.</I></b> Lysate (via sonication) from BL21 E. coli was incubated with Epiprotect (microbial cellulose bandage) for 1h and washed thrice with 70% ethanol.] ]]
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[[File:T--Linkoping Sweden--aspink bunden.jpeg|150px|left|thumb|<b><I>Figure 1.</I></b> Lysate (via sonication) from BL21 E. coli was incubated with Epiprotect (microbial cellulose bandage) for 1h and washed thrice with 70% ethanol.] ]] <b>CBD-asPink bindning capacity</b><br>
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To test the bindning capacity of CBD-asPink (<partinfo>BBa_K3182000</partinfo>) the white microbial cellulose bandage was into the sonicated lysate of BL21(DE3) with the expressed fusion protein (see <b><I>Figure 1</I></b>) and incubated for 30 minutes.
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The bandage was then washed thrice in 70% ethanol which confirmed that the bindning of CDB-asPink was still intact after the washes to the bandage.
  
 
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Revision as of 08:32, 27 August 2019


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Applications of BBa_K1033933

2019 iGEM team Linkoping Sweden

2019 iGEM team Linkoping Sweden validated this part.




Figure 1. Lysate (via sonication) from BL21 E. coli was incubated with Epiprotect (microbial cellulose bandage) for 1h and washed thrice with 70% ethanol.]
CBD-asPink bindning capacity

To test the bindning capacity of CBD-asPink (BBa_K3182000) the white microbial cellulose bandage was into the sonicated lysate of BL21(DE3) with the expressed fusion protein (see Figure 1) and incubated for 30 minutes. The bandage was then washed thrice in 70% ethanol which confirmed that the bindning of CDB-asPink was still intact after the washes to the bandage.



Figure 2. To test the release mechanism, human thrombin and cleavage buffer was added to the bandage and incubated for 16 hours on an end to end rotator.

































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UNIQ029300dfba0bc1fb-partinfo-00000001-QINU UNIQ029300dfba0bc1fb-partinfo-00000002-QINU