Difference between revisions of "Part:BBa K1033933:Experience"

Revision as of 08:16, 27 August 2019

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Applications of BBa_K1033933

2019 iGEM team Linkoping Sweden

2019 iGEM team Linkoping Sweden validated this part.

To test the bindning capacity of CBD-asPink (BBa_K3182000) we placed a white microbial cellulose bandage into the sonicated lysate of BL21(DE3) containing(?) the fusion protein see Figure 1</b> and let it incubate for 30 minutes. The bandage was then washed thrice in 70% ethanol which confirmed that the bindning of CDB-asPink was still intact after the washes to the bandage.

<b>Figure 1.Lysate (via sonication) from BL21 E. coli was incubated with Epiprotect (microbial cellulose bandage) for 1h and washed thrice with 70% ethanol.]

Test of the bindning ability of CBD-asPink (BBa_K3182000) to a bandage made of microbial cellulose in a PBS buffer solution (Figure 1).

Figure 2.

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UNIQc935f0319d2dc1ba-partinfo-00000002-QINU UNIQc935f0319d2dc1ba-partinfo-00000003-QINU

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 iGEM team Linkoping Sweden validated this part.<br><br>
+To test the bindning capacity of CBD-asPink (<partinfo>BBa_K3182000</partinfo>) we placed a white microbial cellulose bandage into the sonicated lysate of BL21(DE3) containing(?) the fusion protein see <b><I> Figure 1</b> </I> and let it incubate for 30 minutes.
+The bandage was then washed thrice in 70% ethanol which confirmed that the bindning of CDB-asPink was still intact after the washes to the bandage.
 <br><br>
-[[File:T--Linkoping Sweden--aspink bunden.jpeg|150px|left|thumb|<b>Figure 1.</b>Lysate (via sonication) from BL21 E. coli was incubated with Epiprotect (microbial cellulose bandage) for 1h and washed thrice with 70% ethanol.] ]]Test of the bindning ability of CBD-asPink (<partinfo>BBa_K3182000</partinfo>) to a bandage made of microbial cellulose in a PBS buffer solution (<I>Figure 1.</I>).
+[[File:T--Linkoping Sweden--aspink bunden.jpeg|150px|left|thumb|<b>Figure 1.</b>Lysate (via sonication) from BL21 E. coli was incubated with Epiprotect (microbial cellulose bandage) for 1h and washed thrice with 70% ethanol.] ]]Test of the bindning ability of CBD-asPink (<partinfo>BBa_K3182000</partinfo>) to a bandage made of microbial cellulose in a PBS buffer solution (<I>Figure 1</I>).
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