Difference between revisions of "Part:BBa K3182300"

Revision as of 12:51, 18 September 2019

pT7-mNG This is a improved variant of BBa_K2671000. This part codes for mNeonGreen, which is a fluorescent protein with great intensity. mNeonGreen has a excitation maximum at 506 nm and a emission maximum at 517, providing a small Stoke's shift. It's color can be seen under normal white light as a yellow-green color.

Figure 1.

T--Linkoping_Sweden--iumprovement123.png

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 91
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K3182300 short</partinfo>
+This is a improved variant of BBa_K2671000. This part codes for mNeonGreen, which is a fluorescent protein with great intensity. mNeonGreen has a excitation maximum at 506 nm and a emission maximum at 517, providing a small Stoke's shift. It's color can be seen under normal white light as a yellow-green color.
-mNeonGreen with a T7 promotor.
+[[File:T--Linkoping_Sweden--iumprovement123.png|420px|thumb|right|<b>Figure 1.</b> ]]
-[[File:T--Linkoping_Sweden--mng-mtfp.png|430px|thumb|left|<b>Figure 1.</b> Photo of this mNG part (BBa_K3182300) after 16h of induction at 16°C over-night. It was expressed in BL21 (DE3) gold with 1mM IPTG. To the right of mNG are mTFP expressed in BL21 and V. Natriegens (Vmax strain).]]
+T--Linkoping_Sweden--iumprovement123.png
-[[File:T--Linkoping_Sweden--mng-mtfp-UV.png|430px|thumb|right|<b>Figure 2.</b> Photo of this mNG part (BBa_K3182300) after 16h of induction at 16°C over-night. This is the same cultures as figure 1 to the left. The cultures were photographed on an UV-table (302nm). It was expressed in BL21 (DE3) gold with 1mM IPTG. To the right of mNG are mTFP expressed in BL21 and V. Natriegens (Vmax strain).]]
 <!-- Add more about the biology of this part here