Difference between revisions of "Part:BBa K3182300"

Revision as of 08:02, 27 August 2019

pT7-mNG

mNeonGreen with a T7 promotor.

Figure 1. Photo of this mNG part (BBa_K3182300) after 16h of induction at 16°C over-night. It was expressed in BL21 (DE3) gold with 1mM IPTG. To the right of mNG are mTFP expressed in BL21 and V. Natriegens (Vmax strain).

Figure 2. Photo of this mNG part (BBa_K3182300) after 16h of induction at 16°C over-night. This is the same cultures as figure 1 to the left. The cultures were photographed on an UV-table (302nm). It was expressed in BL21 (DE3) gold with 1mM IPTG. To the right of mNG are mTFP expressed in BL21 and V. Natriegens (Vmax strain).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 91
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 mNeonGreen with a T7 promotor.
-[[File:T--Linkoping_Sweden--mng-mtfp.png|500px|thumb|center|<b>Figure 3.</b> Photo of this mNG part (BBa_K3182300) after 16h of induction at 16°C over-night. It was expressed in BL21 (DE3) gold with 1mM IPTG. To the right of mNG are mTFP expressed in BL21 and V. Natriegens (Vmax strain).]]
+[[File:T--Linkoping_Sweden--mng-mtfp.png|430px|thumb|left|<b>Figure 1.</b> Photo of this mNG part (BBa_K3182300) after 16h of induction at 16°C over-night. It was expressed in BL21 (DE3) gold with 1mM IPTG. To the right of mNG are mTFP expressed in BL21 and V. Natriegens (Vmax strain).]]
+[[File:T--Linkoping_Sweden--mng-mtfp-UV.png|430px|thumb|right|<b>Figure 2.</b> Photo of this mNG part (BBa_K3182300) after 16h of induction at 16°C over-night. This is the same cultures as figure 1 to the left. The cultures were photographed on an UV-table (302nm). It was expressed in BL21 (DE3) gold with 1mM IPTG. To the right of mNG are mTFP expressed in BL21 and V. Natriegens (Vmax strain).]]
 <!-- Add more about the biology of this part here