Difference between revisions of "Part:BBa K2909000"
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===Usage and Biology===
 
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===Functional Parameters===
 
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Revision as of 15:06, 26 August 2019

HiBiT-B2 MoClo C. reinhardtii

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Introduction

1- Biological background

HiBiT Tag developed by Promega to allow for a quick method of protein quantification by luminescence. This part is standardized in the MoClo standard for Chlamydomonas reinhardtii. This tag is flanked on both side by specific fusion sites and BbsI sites to allow its integration into a level 0 plasmid of the C. reinhardtii MoClo Kit. This tag is designed to be integrated at the B2 position (N-terminal tag).

2- Usage in iGEM projects

Bio[oil]gical Factory (iGEM Sorbonne Université 2019)

Characterization

References