Difference between revisions of "Part:BBa K3174002"
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<partinfo>BBa_K3174002 short</partinfo> | <partinfo>BBa_K3174002 short</partinfo> | ||
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This part has the blue fluorescent protein (mTagBFP) gene inserted downstream of a strong promoter and strong ribosome binding site. | This part has the blue fluorescent protein (mTagBFP) gene inserted downstream of a strong promoter and strong ribosome binding site. | ||
<partinfo>BBa_J23104</partinfo> is part of the Anderson Series of constitutive promoters designed by iGEM2006_Berkeley. It was used by Manchester in 2017 to create <partinfo>BBa_K2213008</partinfo>. | <partinfo>BBa_J23104</partinfo> is part of the Anderson Series of constitutive promoters designed by iGEM2006_Berkeley. It was used by Manchester in 2017 to create <partinfo>BBa_K2213008</partinfo>. | ||
Revision as of 04:49, 19 August 2019
Blue Fluorescent Protein with Strong Promoter and Strong RBS
This part has the blue fluorescent protein (mTagBFP) gene inserted downstream of a strong promoter and strong ribosome binding site. BBa_J23104 is part of the Anderson Series of constitutive promoters designed by iGEM2006_Berkeley. It was used by Manchester in 2017 to create BBa_K2213008.
Usage and Biology
UT Austin iGEM 2019 Team transformed this part into the E.coli DH10B burden monitor (https://2019.igem.org/Team:Austin_UTexas
) and used it as a standard with which cellular burden could be quantified (by means of a GFP expression vs growth rate model) for other BioBricks that had been similarly transformed into the burden strain.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]