Difference between revisions of "Part:BBa K2976008"
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| − | + | ===Usage and Biology=== | |
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| + | Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates the following characteristics: solubility, detection, purification, localization and expression. The HA tag (YPYDVPDYA) is derived from an epitope of the influenza hemagglutinin protein which has been used extensively as a general epitope tag in expression vectors due to its small size and low influence on the tagged protein’s biochemical properties. In 2019 CPU_CHINA project, we insert HA tag into the fusion proteins on the membrane of exosomes for the immunoblotting assay. | ||
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Revision as of 00:44, 5 August 2019
Usage and Biology
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates the following characteristics: solubility, detection, purification, localization and expression. The HA tag (YPYDVPDYA) is derived from an epitope of the influenza hemagglutinin protein which has been used extensively as a general epitope tag in expression vectors due to its small size and low influence on the tagged protein’s biochemical properties. In 2019 CPU_CHINA project, we insert HA tag into the fusion proteins on the membrane of exosomes for the immunoblotting assay.