Difference between revisions of "Part:BBa K2982004"

 
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<partinfo>BBa_K2982004 short</partinfo>
 
<partinfo>BBa_K2982004 short</partinfo>
  
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A coding sequence for mutated W159H/S245I PETase from Ideonella sakainesis which is in BioBrick RFC[10] assembly standard and also contains two site for restriction enzymes, XhoI and BamHI to act on. It is codon optimized for Escherichia coli.
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A coding sequence for mutated W159H/S245I PETase from Ideonella sakainesis which is in BioBrick RFC[10] assembly standard and also contains two sites for restriction enzymes, XhoI and BamHI to act on. It is codon optimized for Escherichia coli.
  
 
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Revision as of 04:45, 7 August 2019


Coding sequence for W159H/S245I IsPETase double mutant

A coding sequence for mutated W159H/S245I PETase from Ideonella sakainesis which is in BioBrick RFC[10] assembly standard and also contains two sites for restriction enzymes, XhoI and BamHI to act on. It is codon optimized for Escherichia coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 348
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 304
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 348
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 348
    Illegal AgeI site found at 627
  • 1000
    COMPATIBLE WITH RFC[1000]