Difference between revisions of "Part:BBa K2916007:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
 +
 
The sequence was extracted from the pET21a-GlyRS-His plasmid and expressed in the BL21 (DE3) E.coli strain.  
 
The sequence was extracted from the pET21a-GlyRS-His plasmid and expressed in the BL21 (DE3) E.coli strain.  
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−
 
  
 
===Source===
 
===Source===
Line 16: Line 15:
  
 
===References===
 
===References===
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 +
Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568

Revision as of 13:32, 24 July 2019


PheRS protein equipped with a 6x HIS affinity tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2424
    Illegal BamHI site found at 31
    Illegal BamHI site found at 1772
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1162
    Illegal AgeI site found at 1155
    Illegal AgeI site found at 2077
    Illegal AgeI site found at 2929
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2875
    Illegal SapI.rc site found at 198
    Illegal SapI.rc site found at 2851


Design Notes

The sequence was extracted from the pET21a-GlyRS-His plasmid and expressed in the BL21 (DE3) E.coli strain.

Source

http://www.addgene.org/124110/

References

Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568