Difference between revisions of "Part:BBa K2916013:Design"

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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K2916003 short</partinfo>
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<partinfo>BBa_K2916012 short</partinfo>
  
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<partinfo>BBa_K2916003 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2916012 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
  
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The sequence was extracted from the pET21a-CysRS-His plasmid and expressed in the BL21 (DE3) E.coli strain.
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The sequence was extracted from the pET21a-AspRS-His plasmid and expressed in the BL21 (DE3) E.coli strain.
  
 
===Source===
 
===Source===

Revision as of 13:00, 24 July 2019


NDK protein eqquiped with a 6x HIS affinity tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 31
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was extracted from the pET21a-AspRS-His plasmid and expressed in the BL21 (DE3) E.coli strain.

Source

http://www.addgene.org/124106/

References

Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568