Difference between revisions of "Part:BBa K2916000:Design"

(→‎References)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
  
−
The protein was cloned in the pQE30 vector and expressed in the M15 E.coli strain
+
The sequence was extracted from the pQE30-His-AlaRS plasmid and expressed in the M15 E.coli strain
  
 
===Source===
 
===Source===

Revision as of 14:58, 23 July 2019


ArgRS protein equipped with a 10x HIS affinity tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 76
    Illegal SapI.rc site found at 1495


Design Notes

The sequence was extracted from the pQE30-His-AlaRS plasmid and expressed in the M15 E.coli strain

Source

Escherichia coli gene alaS

References

Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568