Difference between revisions of "Part:BBa J119440"
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The part forms an i-motif and/or G quadruplex, and has sticky ends on both 5' ends. The i-motif is located on the template strand in this sequence. If the part was to be ligated into a plasmid that had been digested with Eco RI, it could be ligated in either oreintation (i-motif on the coding strand or G-quadruplex on the coding strand). The i-motif/G-quadruplex-forming sequence comes from the human c-Myc promoter's Nuclease Hypersenstive Element III. | The part forms an i-motif and/or G quadruplex, and has sticky ends on both 5' ends. The i-motif is located on the template strand in this sequence. If the part was to be ligated into a plasmid that had been digested with Eco RI, it could be ligated in either oreintation (i-motif on the coding strand or G-quadruplex on the coding strand). The i-motif/G-quadruplex-forming sequence comes from the human c-Myc promoter's Nuclease Hypersenstive Element III. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 21:24, 18 July 2019
c-Myc Promoter G-quadruplex and i-Motif
The part forms an i-motif and/or G quadruplex, and has sticky ends on both 5' ends. The i-motif is located on the template strand in this sequence. If the part was to be ligated into a plasmid that had been digested with Eco RI, it could be ligated in either oreintation (i-motif on the coding strand or G-quadruplex on the coding strand). The i-motif/G-quadruplex-forming sequence comes from the human c-Myc promoter's Nuclease Hypersenstive Element III.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]