Difference between revisions of "Part:BBa K3182100"
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AsPink dropout enabling colour-screening for positive colonies. Using BamHI and SpeI or PstI on both this part assembled in pSB1C3 and the insert of choice will yield a fusion protein between CBDcipA and the insert. The fusion protein can later be cleaved with thrombin to yield two separate proteins. The C-terminal fusion will have one glycine and one serine added to the N-terminal of the protein. | AsPink dropout enabling colour-screening for positive colonies. Using BamHI and SpeI or PstI on both this part assembled in pSB1C3 and the insert of choice will yield a fusion protein between CBDcipA and the insert. The fusion protein can later be cleaved with thrombin to yield two separate proteins. The C-terminal fusion will have one glycine and one serine added to the N-terminal of the protein. | ||
| − | [[File:T--Linkoping_Sweden--pinkwhite.png| | + | [[File:T--Linkoping_Sweden--pinkwhite.png|1000px|thumb|center|<b>Figure 1.</b> ]] |
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Revision as of 13:24, 14 July 2019
pT7-CBDcipA-pCons-AsPink
AsPink dropout enabling colour-screening for positive colonies. Using BamHI and SpeI or PstI on both this part assembled in pSB1C3 and the insert of choice will yield a fusion protein between CBDcipA and the insert. The fusion protein can later be cleaved with thrombin to yield two separate proteins. The C-terminal fusion will have one glycine and one serine added to the N-terminal of the protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 592
Illegal NheI site found at 615 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 580
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]