Difference between revisions of "Part:BBa K3182000"

 
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A cellulose binding domain which can be used to purify or attach proteins to cellulose. The part also has a flexible GS-linker with a thrombin site added. The part has a AsPink fused to the cellulose binding domain to ensure easy trackable expression and characterization. The part has the strongest available expression system with a T7 promotor and 5'-UTR region as well as other translational enhancers.
 
A cellulose binding domain which can be used to purify or attach proteins to cellulose. The part also has a flexible GS-linker with a thrombin site added. The part has a AsPink fused to the cellulose binding domain to ensure easy trackable expression and characterization. The part has the strongest available expression system with a T7 promotor and 5'-UTR region as well as other translational enhancers.
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[[File:T--Linkoping_Sweden--CBD-AsPink.jpeg|430px|thumb|center|<b>Figure 1.</b> E. coli BL21 cells expressing this biobrick, incubated for 16 hours at 16 degrees Celsius.]]
  
 
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===Usage and Biology===
 
===Usage and Biology===
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Revision as of 11:54, 8 July 2019


pT7-CBDcipA-AsPink

A cellulose binding domain which can be used to purify or attach proteins to cellulose. The part also has a flexible GS-linker with a thrombin site added. The part has a AsPink fused to the cellulose binding domain to ensure easy trackable expression and characterization. The part has the strongest available expression system with a T7 promotor and 5'-UTR region as well as other translational enhancers.

Figure 1. E. coli BL21 cells expressing this biobrick, incubated for 16 hours at 16 degrees Celsius.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 580
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]