Difference between revisions of "Part:BBa K3182000"
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A cellulose binding domain which can be used to purify or attach proteins to cellulose. The part also has a flexible GS-linker with a thrombin site added. The part has a AsPink fused to the cellulose binding domain to ensure easy trackable expression and characterization. The part has the strongest available expression system with a T7 promotor and 5'-UTR region as well as other translational enhancers. | A cellulose binding domain which can be used to purify or attach proteins to cellulose. The part also has a flexible GS-linker with a thrombin site added. The part has a AsPink fused to the cellulose binding domain to ensure easy trackable expression and characterization. The part has the strongest available expression system with a T7 promotor and 5'-UTR region as well as other translational enhancers. | ||
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| + | [[File:T--Linkoping_Sweden--CBD-AsPink.jpeg|430px|thumb|center|<b>Figure 1.</b> E. coli BL21 cells expressing this biobrick, incubated for 16 hours at 16 degrees Celsius.]] | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Revision as of 11:54, 8 July 2019
pT7-CBDcipA-AsPink
A cellulose binding domain which can be used to purify or attach proteins to cellulose. The part also has a flexible GS-linker with a thrombin site added. The part has a AsPink fused to the cellulose binding domain to ensure easy trackable expression and characterization. The part has the strongest available expression system with a T7 promotor and 5'-UTR region as well as other translational enhancers.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 580
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]