Difference between revisions of "Part:BBa K2905000"
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<partinfo>BBa_K2905000 short</partinfo> | <partinfo>BBa_K2905000 short</partinfo> | ||
| − | Trimethylamine methyltransferase. Requires pyrrolysine to be added to stop codon within the open reading frame. | + | Trimethylamine methyltransferase. Requires pyrrolysine to be added to stop codon within the open reading frame. |
| + | |||
| + | Starts with a His-6 tag, followed by a NheI site scar and a TEV protease recognition site, fused in frame to the MttB gene. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 15:07, 24 June 2019
MttB
Trimethylamine methyltransferase. Requires pyrrolysine to be added to stop codon within the open reading frame.
Starts with a His-6 tag, followed by a NheI site scar and a TEV protease recognition site, fused in frame to the MttB gene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 43
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1159
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 808
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 985