Difference between revisions of "Part:BBa K145150:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
−
-35 and -10 boxes have been modified to lower the basal transcription rate
+
-35 and -10 boxes have been modified to lower the basal transcription rate. This part was constructing with an end-filling PCR reaction (with Klenow polymerase) of the following primers:
 +
primer 1: CATCATGAAT TCGCGGCCGC TTCTAGACCT GTAGGATCGT ACAGGTTTAC TAAAGATTCC TTTAG
 +
primer 2: TACTAGTAGC GGCCGCTGCA GGAAATTAAA GAACACTTAA ATTATAAACT AAAGGAATCT TTAG
  
 
===Source===
 
===Source===

Latest revision as of 12:39, 14 October 2008

Hybrid promoter: HSL-LuxR activated, P22 C2 repressed


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-35 and -10 boxes have been modified to lower the basal transcription rate. This part was constructing with an end-filling PCR reaction (with Klenow polymerase) of the following primers: 

primer 1: CATCATGAAT TCGCGGCCGC TTCTAGACCT GTAGGATCGT ACAGGTTTAC TAAAGATTCC TTTAG
primer 2: TACTAGTAGC GGCCGCTGCA GGAAATTAAA GAACACTTAA ATTATAAACT AAAGGAATCT TTAG

Source

Synthetic

References