Difference between revisions of "Part:BBa K2624007:Experience"

Latest revision as of 03:53, 8 December 2018

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2624007

We used this part to construct the "p-H*" plasmid and "p-H*-U6" plasmid, in order to verify the role of the RNA interference caused by pri-miRNA. Through qPCR experiment, it can be seen that pri-miRNA can successfully expressed and processed into miRNA.

20181017210154%21T--CPU_CHINA--hp-qidongzi.png

Figure. The expression of miRNA(MAP4K4)

"p-H*" contains "HULC--pri-miRNA" parts; "p-H*-U6" contains "HULC-pri-miRNA-U6-pSil" parts;

User Reviews

UNIQcff62dc986d85511-partinfo-00000000-QINU UNIQcff62dc986d85511-partinfo-00000001-QINU

@@ Line 5: / Line 5: @@
 ===Applications of BBa_K2624007===
-We used this part to construct the "p-H*" plasmid and "p-H*-U6" plasmid, in order to verify the role of the RNA interference caused by pri-miRNA. Through qPCR experiment, it can be seen that the miRNA expression level of MAP4K4 is increased.
+We used this part to construct the "p-H*" plasmid and "p-H*-U6" plasmid, in order to verify the role of the RNA interference caused by pri-miRNA. Through qPCR experiment, it can be seen that pri-miRNA can successfully expressed and processed into miRNA.
 https://static.igem.org/mediawiki/2018/archive/5/56/20181017210154%21T--CPU_CHINA--hp-qidongzi.png