Difference between revisions of "Part:BBa K2624005:Experience"

(→‎Applications of BBa_K2624005)
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Figure 1. The expression of MAP4K4 mRNA
 
Figure 1. The expression of MAP4K4 mRNA
  
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"p-H" is "pcDNA-HULC--primiRNA(MAP4K4)" plasmid;"Plasmid1" is"pIRES-tTA-NS5B-NLS";"Plasmid2" is"pcDNA-HULC--primiRNA(MAP4K4)-U6-pSil-TRE-sponge".
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"p-H" contains "HULC--pri-miRNA(MAP4K4)" parts; "Plasmid1" contains "tTA-NS5B-NLS" parts; "Plasmid2" contains "HULC--pri-miRNA(MAP4K4)-U6-pSil-TRE-sponge" parts.
  
 
https://static.igem.org/mediawiki/2018/archive/5/56/20181017212106%21T--CPU_CHINA--hp-qidongzi.png
 
https://static.igem.org/mediawiki/2018/archive/5/56/20181017212106%21T--CPU_CHINA--hp-qidongzi.png

Latest revision as of 03:48, 8 December 2018


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2624005

We used this part to construct the "p-H" Plasmid and "Plasmid2", in order to verify the role of RNA interference caused by pri-miRNA. According to the results of qPCR, the decreased MAP4K4 mRNA expression was observed. What's more, the results of MTT shows that the regulatory system we built can work.

20181017211354%21T--CPU_CHINA--hp-qidongzi.png

Figure 1. The expression of MAP4K4 mRNA

"p-H" contains "HULC--pri-miRNA(MAP4K4)" parts; "Plasmid1" contains "tTA-NS5B-NLS" parts; "Plasmid2" contains "HULC--pri-miRNA(MAP4K4)-U6-pSil-TRE-sponge" parts.

20181017212106%21T--CPU_CHINA--hp-qidongzi.png

Figure 2. The MTT Assay

"Plasmid1" contains "tTA-NS5B-NLS" parts; "Plasmid2" contains "HULC--primiRNA(MAP4K4)-U6-pSil-TRE-sponge" parts; TC is the abbreviations of Tetracycline.

User Reviews

UNIQe7ee112adcd5f839-partinfo-00000000-QINU UNIQe7ee112adcd5f839-partinfo-00000001-QINU