Difference between revisions of "Part:BBa K2700001"
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2700001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2700001 SequenceAndFeatures</partinfo>
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pT181-antisense
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The pT181-antisense transcript interacts with the 5’ untranslated region of the pT181-promoter, inducing a conformational chance leading to transcriptional and translation repression, as seen in figure 2 [2].
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1.    Novick, R.P., et al., pT181 plasmid replication is regulated by a countertranscript-driven transcriptional attenuator. Cell, 1989. 59(2): p. 395-404.
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2.    Westbrook, A.M. and J.B. Lucks, Achieving large dynamic range control of gene expression with a compact RNA transcription–translation regulator. Nucleic Acids Research, 2017. 45(9): p. 5614-5624.
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 03:28, 18 October 2018


Antisense-RNA for transcriptional/translational repression

This is our pt181-antisense-RNA transcript that will block both transcription and translation at the pt181 promoter (Part:BBa_K2700002) site. Once pt181-antisense is expressed, it will bind to the promoter region and cause a physical fold to occur. This fold is a stem-like structure that will block the RBS as well as act as a terminator to prevent further transcription to occur.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

pT181-antisense The pT181-antisense transcript interacts with the 5’ untranslated region of the pT181-promoter, inducing a conformational chance leading to transcriptional and translation repression, as seen in figure 2 [2].

1. Novick, R.P., et al., pT181 plasmid replication is regulated by a countertranscript-driven transcriptional attenuator. Cell, 1989. 59(2): p. 395-404. 2. Westbrook, A.M. and J.B. Lucks, Achieving large dynamic range control of gene expression with a compact RNA transcription–translation regulator. Nucleic Acids Research, 2017. 45(9): p. 5614-5624.