Difference between revisions of "Part:BBa K2588020"
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<p>The outer membrane protease T is a housekeeping protease. It degrades peptides in the close environment of the bacteria.</p>
  
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The outer membrane protease T is a housekeeping protease. It degrades peptides in the close environment of the bacteria.
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<h2>Usage and Biology</h2>
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===Usage and Biology===
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OmpT can be used to facilitate protein export by clevage of the OmpTSite bound to a target protein. It is a potential probe for mass spectrometry.
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<p>OmpT can be used to facilitate protein export by clevage of the OmpTSite bound to a target protein. This way we use it to cleave the signal peptide <a href="https://parts.igem.org/Part:BBa_K554002">HlyA</a></p> from the insecticide <a href="https://parts.igem.org/Part:BBa_K2588019">BjaIT</a></p>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 03:21, 18 October 2018


Outer membrane protease T

The outer membrane protease T is a housekeeping protease. It degrades peptides in the close environment of the bacteria.

Usage and Biology

OmpT can be used to facilitate protein export by clevage of the OmpTSite bound to a target protein. This way we use it to cleave the signal peptide HlyA

from the insecticide BjaIT

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]