Difference between revisions of "Part:BBa K2680071"

Revision as of 01:52, 18 October 2018

39C Temperature-Sensitive (ptplA) mScarlet-I pdt#3e

Components: J23107 TPL + ptlpA b0034m mScarlet-I pdt#3e

Mechanics

J23107, a medium-weak constitutive promoter, constitutively expresses TlpA39. Unlike the original TlpA, TlpA39 undergoes a conformational change at 39C and loses its ability to bind the downstream pTlpA. Therefore, TlpA39 halts its repression of promoter pTlpA at temperatures above 39C, inducing the expression of the rapidly-maturing mScarlet-I. The mScarlet-I in this circuit has a pdt degradation tag, allowing for degradation by the mf-Lon protease. Specifically, this circuit contains pdt#3e, a weak and slow degradation tag from part series K2333401-K2333406 or BBa_K2333406.

As an IFFL

In this circuit, heat activates a reporter, mScarlet-I. In a complete IFFL (incoherent feed forward loop), an input must activate both a reporter and its inhibitor. Therefore, this circuit requires mf-Lon (or a different inhibitor of mScarlet-I) to act as an IFFL.

Sequence and Features