Difference between revisions of "User:Scmohr/Antibiotic-res cat page"
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These are BioBrick composite parts that contain (one or more?) antibiotic resistance components. | These are BioBrick composite parts that contain (one or more?) antibiotic resistance components. | ||
| − | <SPAN style= 'color:red'>'''Note: Antibiotic resistance genes are present in ''most'' of the plasmids (see | + | <SPAN style= 'color:red'>'''Note: Antibiotic resistance genes are present in ''most'' of the plasmids (see [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=Plasmid Plasmid] and '''</span> |
Revision as of 17:18, 30 July 2008
Antibiotic Resistance Basic Parts
Introduction to Antibiotic Resistance
The established mechanisms of antibiotic resistance include the following:
- Enzymes that inactivate the antibiotic [famously (a). beta-lactamases that destroy penicillins and related compounds and (b) enzymes that acetylate chloramphenicol].
- Proteins that keep antibiotics out of the cell (by blocking the outer-membrane pores).
- Membrane-embedded channel proteins that actively pump antibiotics out of the cell.
- Proteins (and RNAs) with altered drug binding sites. (Vancomycin resistance comes from a switch in the chemistry of the cell-wall cross-linking peptide). This category also includes mutations in ribosomal RNA -- ribosomes are one of the main targets of both prokaryote- and eukaryote-directed antibiotics.
- Proteins that enable substitution of an alternative metabolic pathway (as in the case of sulfonamide resistance).
Parts by Category
NB Parts listed in boldface are "good" parts ["Available" and (in most cases) "Work"]. Parts in plain type are poorly characterized.
1. Antibiotic-inactivating Enzymes
- Basic parts
- BBa_J23012
- BBa_J31002
- BBa_J31003
- BBa_J31004
- BBa_J31005
- BBa_J42020
- BBa_J42021
- Plasmids
- BBa_K125000
2. Membrane-blocking Proteins
3. Membrane-embedded Efflux Pumps
- Basic parts
- BBa_J31006
- BBa_J31007
4. Altered Target Molecules
- Basic parts
- BBa_J42010
5. Components of Alternative Metabolic Pathways
Antibiotic Resistance Devices
These are BioBrick composite parts that contain (one or more?) antibiotic resistance components.
Note: Antibiotic resistance genes are present in most of the plasmids (see Plasmid and
Useful Information about Antibiotic Resistance
Links
Aminoglycoside Resistance
This category includes kanamycin, streptomycin, gentamycin, neomycin, tobramycin, amikacin...
http://www.antibioresistance.be/aminoglycosides.html
http://openwetware.org/wiki/Kanamycin
Tetracycline Resistance
This category includes three different resistance mechanisms. Related antibiotics are tetracycline, chlortetracycline, doxycycline, minocycline, oxytetracycline, spectinomycin. Note that the TetR gene encodes a very popular repressor that is frequently used in synthetic biology simply as a single control element in cells that do not express tetracycline resistance. [Check this.]
http://www.antibioresistance.be/Tetracycline/Menu_Tet.html
http://openwetware.org/wiki/Tetracycline
http://cmr.asm.org/cgi/content/abstract/5/4/387