Difference between revisions of "Part:BBa K2560131:Design"

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===Design Notes===
 
===Design Notes===
  
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The promoter dummy was designed with a high GC-content to reduce propability of transcription initiation.
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The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector <a href="https://parts.igem.org/Part:BBa_K2560002">BBa_K2560002</a> using Golden Gate assembly. If you stuggle with <i> de novo </i> synthesis we recomended this
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<a href="https://parts.igem.org/Help:Promoters/Construction">site</a>.  
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<b> Forward oligo:</b>
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CTCGGGAGCCCCTGGCGCCCCTTTACT
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<b> Reverse Oligo:</b>
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CTCAAGTAAAGGGGCGCCAGGGGCTCC
  
 
===Source===
 
===Source===
  
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More information coming soon!
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===References===
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As Source DNA we used PYTK from the Dueber Toolbox. <a href="https://pubs.acs.org/doi/abs/10.1021/sb500366v"><abbr title="A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly">(Lee <i>et al.</i> 2015.)</abbr>  </a>
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===Source===
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The promoter dummy was designed with a high GC-content to reduce propability of transcription initiation.

Revision as of 23:59, 17 October 2018


Phytobrick version of Promoter Dummy


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly. If you stuggle with de novo synthesis we recomended this site.

Forward oligo: CTCGGGAGCCCCTGGCGCCCCTTTACT

Reverse Oligo: CTCAAGTAAAGGGGCGCCAGGGGCTCC

Source

As Source DNA we used PYTK from the Dueber Toolbox. (Lee et al. 2015.)


Source

The promoter dummy was designed with a high GC-content to reduce propability of transcription initiation.