Difference between revisions of "Part:BBa K2787016"
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | A plasmid under control of B8 orthogonal RBS is constructed with the C9 orthogonal ribosome. | ||
[[File:Shanghaitech2018-oribo-orbs-07-07.png|700px|center]] | [[File:Shanghaitech2018-oribo-orbs-07-07.png|700px|center]] | ||
[[File:ShanghaiTech2018-b8 rbs.png|700px|center]] | [[File:ShanghaiTech2018-b8 rbs.png|700px|center]] |
Latest revision as of 03:59, 18 October 2018
GFP under B8 orthogonal RBS with orthogonal 16S rRNA C9
The 16S rRNA C9 under constitutive promoter BBa_J23100(BBa_K2787007) and pCons-oRBSB8-GFP(BBa_K2787001) are linked into one plasmid to quantify the orthogonality of the C9 and B8 ribosome system.
Usage and Biology
A plasmid under control of B8 orthogonal RBS is constructed with the C9 orthogonal ribosome.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 798
Illegal NheI site found at 821 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1660
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 707