Difference between revisions of "Part:BBa K2859002:Experience"
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| − | We synthesised the part flanked by bioBrick prefix and suffix. Then, the synthesized gblock was ligated into pSB1C3 by restriction digest with EcoR1 and Pst1 and transformed into E.Coli DH5-α.The transformed E.coli was grown at 37°C. The expression of Penetratin-Insulin-Flag tag complex was evaluated by SDS page gel. | + | We synthesised the part flanked by bioBrick prefix and suffix. Then, the synthesized gblock was ligated into pSB1C3 by restriction digest with EcoR1 and Pst1 and transformed into E.Coli DH5-α.We confirmed that the ligation was successfully done by running gel with minicprepped DNA cut with EcoR1 (about 2.4kb). The transformed E.coli was grown at 37°C. The expression of Penetratin-Insulin-Flag tag complex was evaluated by SDS page gel. |
| − | + | Figure1. 1.2% Agarose Gel electrophoresis result of pSB1C3 cut with EcoR1 | |
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We synthesised the part flanked by bioBrick prefix and suffix. Then, the synthesized gblock was ligated into pSB1C3 by restriction digest with EcoR1 and Pst1 and transformed into E.Coli DH5-α.We confirmed that the ligation was successfully done by running gel with minicprepped DNA cut with EcoR1 (about 2.4kb). The transformed E.coli was grown at 37°C. The expression of Penetratin-Insulin-Flag tag complex was evaluated by SDS page gel.
Figure1. 1.2% Agarose Gel electrophoresis result of pSB1C3 cut with EcoR1