Difference between revisions of "Part:BBa K2560060:Design"
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− | This part was created by polymerase chain reaction (PCR) on the template <a href="https://parts.igem.org/Part:BBa_K2560033"> | + | This part was created by polymerase chain reaction (PCR) on the template <a href="https://parts.igem.org/Part:BBa_K2560033">BBa_K2560033</a> and subsequent integration into the part entry vector <a href="https://parts.igem.org/Part:BBa_K2560002">BBa_K2560002</a> using Golden Gate assembly. |
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Revision as of 22:53, 17 October 2018
Phytobrick version of 4x BBa_E1010
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 552
Illegal AgeI site found at 664 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was created by polymerase chain reaction (PCR) on the template BBa_K2560033 and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly.
Forward oligo: AACGTCTCGCTCGAATGGCTTCCTCCGAAGACGTTATC
Reverse Oligo: TTCGTCTCCCTCACATCCCAGCACCGGTGGAGTGACGAC
Source
As Source DNA we used BBa_BBa_K2560033 from our Toolbox.