Difference between revisions of "Part:BBa K2859000:Experience"
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| − | + | We synthesised the part flanked by bioBrick prefix and suffix. Then, the synthesized gblock was ligated into pSB1C3 by restriction digest with EcoR1 and Pst1 and transformed into E.Coli DH5-α.The transformed E.coli was grown at 37°C. The expression of Penetratin-Insulin-Flag tag complex was evaluated by SDS page gel. | |
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Revision as of 22:47, 17 October 2018
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2859000
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UNIQ4140e4bc73239af0-partinfo-00000000-QINU UNIQ4140e4bc73239af0-partinfo-00000001-QINU
We synthesised the part flanked by bioBrick prefix and suffix. Then, the synthesized gblock was ligated into pSB1C3 by restriction digest with EcoR1 and Pst1 and transformed into E.Coli DH5-α.The transformed E.coli was grown at 37°C. The expression of Penetratin-Insulin-Flag tag complex was evaluated by SDS page gel.
