Difference between revisions of "Part:BBa K2765021:Design"

Line 4: Line 4:
  
 
   
 
   
 +
 
[[Image: T--BIT-China--iGEM2018-Partsregulator-5.png |center|600px|]]
 
[[Image: T--BIT-China--iGEM2018-Partsregulator-5.png |center|600px|]]
  
 
[[Image: T--BIT-China--iGEM2018-Partsregulator-6.png |center|600px|]]
 
[[Image: T--BIT-China--iGEM2018-Partsregulator-6.png |center|600px|]]

Revision as of 23:07, 17 October 2018

To determine whether overexpression of yno1 can induce the endogenous ROS accumulation in yeast, we constructed an expression plasmid based on pESC-Leu, in which the cloned yno1 is driven by GAL1 promoter, and thus the target gene is induced by galactose and repressed by glucose.

T--BIT-China--iGEM2018-Partsregulator-4.png


T--BIT-China--iGEM2018-Partsregulator-5.png
T--BIT-China--iGEM2018-Partsregulator-6.png