Difference between revisions of "Part:BBa K2725013"
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Revision as of 22:18, 17 October 2018
P1003 Casette with 10 Amber substitutions
P1003 Kanamycin resistance cassette with 10 serine codons in the coding sequence substituted for amber stop codons (TAG): S17Amb, S36Amb, S60Amb, S116Amb, S133Amb, S154Amb, S189Amb, S200Amb, S240Amb, S242Amb. When transformed into E .coli there is no P1003 expression and no kanamycin resistance showing that 10 amber substitutions effectively prevents expression.
<img src="http://2018.igem.org/File:T--Edinburgh_UG--prob_per_stop.png" width=“10%" height="10%" align="left">
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]