Difference between revisions of "Part:BBa K2587008"

Latest revision as of 21:49, 17 October 2018

guaD

guaD encodes an enzyme called guanine deaminase which converts guanine to xanthine and ammonia. It can be used as the second step in the degradation of melamine to ammonia, as shown by Shaw et al. 2016¹. In this pathway, it is able to convert ammeline to ammelide.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 11
Illegal SpeI site found at 18
Illegal SpeI site found at 1371
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 18
Illegal SpeI site found at 1371
Illegal NotI site found at 2
Illegal NotI site found at 1378
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 11
Illegal SpeI site found at 18
Illegal SpeI site found at 1371
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 11
Illegal SpeI site found at 18
Illegal SpeI site found at 1371
Illegal AgeI site found at 840
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 25
Illegal BsaI.rc site found at 1364

References

(1) Shaw, A. Joe, et al. "Metabolic engineering of microbial competitive advantage for industrial fermentation processes." Science 353.6299 (2016): 583-586.
http://science.sciencemag.org/content/353/6299/583?casa_token=_q3c5eJ3Gv4AAAAA:3HmgrxE_r8XSnR8YUtF3cLZ9I-luClCJEJcwxy6E_J711AgFcrN7i-ozHCnV_HZQmxteX12dXxw5rMc

@@ Line 21: / Line 21: @@
 <p><strong><h4>References</h4></strong></p>
-<p><strong>(1)</strong>Shaw, A. Joe, et al. "Metabolic engineering of microbial competitive advantage for industrial fermentation processes." Science 353.6299 (2016): 583-586.<br>
+<p><strong>(1)</strong> Shaw, A. Joe, et al. "Metabolic engineering of microbial competitive advantage for industrial fermentation processes." Science 353.6299 (2016): 583-586.<br>
 http://science.sciencemag.org/content/353/6299/583?casa_token=_q3c5eJ3Gv4AAAAA:3HmgrxE_r8XSnR8YUtF3cLZ9I-luClCJEJcwxy6E_J711AgFcrN7i-ozHCnV_HZQmxteX12dXxw5rMc</p>