Difference between revisions of "Part:BBa K2587000"
 
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<partinfo>BBa_K2587000 short</partinfo>
 
<partinfo>BBa_K2587000 short</partinfo>
  
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LuxI is an acyl homoserine lactone synthase, most known from the bacterium <i>Aliivibrio fischeri</i>. LuxI is a basic component of the quorum sensing system of <i>A. fischeri</I>. Bacteria use it as a communication module  to regulate expression of genes in a cell density-dependent manner. This part contains a variant of the synthase codon optimized for the common yeast, <i>Saccharomyces cerevisiae</I>. Besides the codon optimization, the construct contains also type IIS restriction sites (<i>Bsa</i>I), useful for example for the Golden Gate assembly cloning method. Since yeast is a eukaryotic organism and the quorum sensing system is encountered in prokaryotes, a codon optimized variant for utilization in an eukaryotic organism represents the beginning of designing quorum sensing-based control systems in eukaryotes. For example, LuxI is coupled with the design of a synthetic promoter, which is supposed to be activated by binding of the quorum sensing molecule together with the regulator (LuxR) to the promoter to induce expression of a reporter gene or a lysis gene to control cell population.  
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LuxI is an acyl homoserine lactone synthase, most known from the bacterium <i>Aliivibrio fischeri</i>. LuxI is a basic component of the quorum sensing system of <i>A. fischeri</I>. Bacteria use it as a communication module  to regulate expression of genes in a cell density-dependent manner. This part contains a variant of this synthase codon optimized for the common yeast, <i>Saccharomyces cerevisiae</I>. Besides the codon optimization, the construct also contains type II S restriction sites (<i>Bsa</i>I), useful for example for the Golden Gate assembly cloning method. Since yeast is a eukaryotic organism and the quorum sensing system is encountered in prokaryotes, a codon optimized variant for utilization in an eukaryotic organism represents the beginning of designing quorum sensing-based control systems in eukaryotes. For example, LuxI is coupled with the design of a synthetic promoter, which is supposed to be activated by binding of the quorum sensing molecule together with the regulator (LuxR) to the promoter to induce expression of a reporter gene or a lysis gene to control cell population.  
  
  

Latest revision as of 23:01, 17 October 2018


luxI_codon optimized S.cerevisiae

LuxI is an acyl homoserine lactone synthase, most known from the bacterium Aliivibrio fischeri. LuxI is a basic component of the quorum sensing system of A. fischeri. Bacteria use it as a communication module to regulate expression of genes in a cell density-dependent manner. This part contains a variant of this synthase codon optimized for the common yeast, Saccharomyces cerevisiae. Besides the codon optimization, the construct also contains type II S restriction sites (BsaI), useful for example for the Golden Gate assembly cloning method. Since yeast is a eukaryotic organism and the quorum sensing system is encountered in prokaryotes, a codon optimized variant for utilization in an eukaryotic organism represents the beginning of designing quorum sensing-based control systems in eukaryotes. For example, LuxI is coupled with the design of a synthetic promoter, which is supposed to be activated by binding of the quorum sensing molecule together with the regulator (LuxR) to the promoter to induce expression of a reporter gene or a lysis gene to control cell population.


Usage and Biology

This part can be used as a component for designing synthetic circuits in S.cerevisiae. For instance, in our project, we used this gene to induce expression of a reporter after activation of a synthetic, self designed promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 6
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 689
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 30
    Illegal BsaI.rc site found at 673