Difference between revisions of "Part:BBa K2587005"

Revision as of 21:15, 17 October 2018

lasR

LasR is the regulator of the quorum sensing system from Pseudomonas aeruginosa. In this case the sequence is codon optimised for E. coli and together with LasI and P_las is supposed to induce expression of a gene of interest in a population density dependent manner.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 579
Illegal SpeI site found at 738
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 579
Illegal SpeI site found at 738
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 579
Illegal SpeI site found at 738
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 579
Illegal SpeI site found at 738
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 731

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 <partinfo>BBa_K2587005 short</partinfo>
-LasR is the regulator of the quorum sensing system from <i>Pseudomonas aeruginosa</i>. In this case the sequence is codon optimized for <i>E. coli</i>. It works as a repressor for P<i><sub>las</sub></i>; upon binding of the corresponding quorum sensing molecule, the promoter is released and is able to induce expression of a gene of interest in a population density dependent manner.
+LasR is the regulator of the quorum sensing system from <i>Pseudomonas aeruginosa</i>. In this case the sequence is codon optimised for <i>E. coli</i> and together with LasI and P<i><sub>las</sub></i> is supposed to induce expression of a gene of interest in a population density dependent manner.
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