Difference between revisions of "Part:BBa K2685007:Design"

Revision as of 20:20, 17 October 2018

Detecting parts

This part is our part of the test to detect tetracycline in the environment. Because E. coli is not tolerant to tetracycline,

we introduced a resistance mechanism to expand its tolerance to tetracycline, which can be detected at a wider concentration

of tetracycline. The ABS value of E. coli was determined by comparing with that of E.coli. The results showed that the highest

concentration of tetracycline was 10μg/ml in E.coli and 110μg /ml in E.coli.

""

The following picture shows the contents of NKU_China’s and our collaboration. Repeated two experiments at 0.2% inoculation

level, the MIC value of detection parts can also be measured to show that detection parts are tolerable.

""

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1768
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1622
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1068
Illegal NgoMIV site found at 1228
Illegal NgoMIV site found at 1596
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 69

@@ Line 8: / Line 8: @@
    <div class="at-user__avatar" align="center">
 "https://static.igem.org/mediawiki/parts/e/e6/T--TUST_China--part_document_072.png"
+                    </div>
+<p>The following picture shows the contents of NKU_China’s and our collaboration. Repeated two experiments at 0.2% inoculation </p>
+<p>level, the MIC value of detection parts can also be measured to show that detection parts are tolerable.</p>
+<div class="at-user__avatar" align="center">
+"https://static.igem.org/mediawiki/parts/7/7c/T--TUST_China--MIC.png"
                      </div>
 <partinfo>BBa_K2685007 SequenceAndFeatures</partinfo>