Difference between revisions of "Part:BBa K2624000:Experience"
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===Applications of BBa_K2624000===
 
===Applications of BBa_K2624000===
 
To improve the selectivity of our therapy system, we utilize hTERT, the cancer-specific promoter, and HULC, the HCC-specific promoter, to open an AND-gated system to target HCC (Hepatocellular carcinoma). pGL3-Basic vector, a promoterless vector is used for measuring the activity of promoter with a luciferase assay. We determined the activity of the two promoters by it. Experimental results show that the promoter has a good startup efficiency.
 
To improve the selectivity of our therapy system, we utilize hTERT, the cancer-specific promoter, and HULC, the HCC-specific promoter, to open an AND-gated system to target HCC (Hepatocellular carcinoma). pGL3-Basic vector, a promoterless vector is used for measuring the activity of promoter with a luciferase assay. We determined the activity of the two promoters by it. Experimental results show that the promoter has a good startup efficiency.
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"https://static.igem.org/mediawiki/2018/5/56/T--CPU_CHINA--hp-qidongzi.png"
 
"https://static.igem.org/mediawiki/2018/5/56/T--CPU_CHINA--hp-qidongzi.png"
  

Revision as of 20:15, 17 October 2018


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Applications of BBa_K2624000

To improve the selectivity of our therapy system, we utilize hTERT, the cancer-specific promoter, and HULC, the HCC-specific promoter, to open an AND-gated system to target HCC (Hepatocellular carcinoma). pGL3-Basic vector, a promoterless vector is used for measuring the activity of promoter with a luciferase assay. We determined the activity of the two promoters by it. Experimental results show that the promoter has a good startup efficiency.

"T--CPU_CHINA--hp-qidongzi.png"

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