Difference between revisions of "Part:BBa K2719000:Experience"
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<p>To prove the presence of the plasmid inside the colonies, two agarose gels were elaborated, one for watch the entire plasmid and the second one for the plasmid with a previously restriction process using NotI, so the last one could confirm the presence of GST. (Figure 2 and 3)</p> | <p>To prove the presence of the plasmid inside the colonies, two agarose gels were elaborated, one for watch the entire plasmid and the second one for the plasmid with a previously restriction process using NotI, so the last one could confirm the presence of GST. (Figure 2 and 3)</p> | ||
[[file:T--TecCEM--GelK2719000.png|500px]] | [[file:T--TecCEM--GelK2719000.png|500px]] | ||
| − | <p><i>Figure 2.</i> 0.85% agarose gel with GelRed. MW) NEB 1Kb Plus DNA Ladder, 13)Extraction GST BBa_K2719000, 14) Extraction GST BBa_K2719000 duplicate</p> | + | <p><i>Figure 2.</i> 0.85% agarose gel with GelRed. MW) NEB 1Kb Plus DNA Ladder, 13)Extraction GST BBa_K2719000, 14) Extraction GST BBa_K2719000 duplicate</p> |
| + | [[file:T--TecCEM--GelK2719000R.png|500px]] | ||
| + | <p><i>Figure 3.</i> GST restriction with NotI in a 0.85% agarose gel with GelRed. MW) 2-Log DNA Ladder, 7)Extraction GST (BBa_K2719000)</p> | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 22:17, 17 October 2018
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K2719000
To confirm the presence of GST, it was cloned in pSB1C3 using EcoRI and PstI for the restriction and T4 ligase for the ligation. After that, it was transformed in Escherichia coli DH5a. (Figure 1)
Figure 1. Transformed GST Colonies
To prove the presence of the plasmid inside the colonies, two agarose gels were elaborated, one for watch the entire plasmid and the second one for the plasmid with a previously restriction process using NotI, so the last one could confirm the presence of GST. (Figure 2 and 3)
Figure 2. 0.85% agarose gel with GelRed. MW) NEB 1Kb Plus DNA Ladder, 13)Extraction GST BBa_K2719000, 14) Extraction GST BBa_K2719000 duplicate
Figure 3. GST restriction with NotI in a 0.85% agarose gel with GelRed. MW) 2-Log DNA Ladder, 7)Extraction GST (BBa_K2719000)
User Reviews
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