Difference between revisions of "Part:BBa R0040"
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From the reference article:<blockquote> | From the reference article:<blockquote> | ||
"In contrast to tetracycline, anhydrotetracycline is a particularly useful inducer. It binds Tet R with an ~35-fold higher binding constant and thus allows to operate at very low concentrations. At the same time, its antibiotic activity is ~100-fold lower and concentrations of <50 ng/ml as required for the full induction of P LtetO-1 have no effect on the growth of E.coli."</blockquote> | "In contrast to tetracycline, anhydrotetracycline is a particularly useful inducer. It binds Tet R with an ~35-fold higher binding constant and thus allows to operate at very low concentrations. At the same time, its antibiotic activity is ~100-fold lower and concentrations of <50 ng/ml as required for the full induction of P LtetO-1 have no effect on the growth of E.coli."</blockquote> | ||
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| + | ===Improved by Fudan-CHINA in 2018=== | ||
| + | This year, Fudan-CHINA makes a mutation to Part:BBa_R0040 to improve its combination with the tTA transcriptional factor by computational simulation.Please be free to visit [[Part:BBa_K2886010]]. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_R0040 SequenceAndFeatures</partinfo> | <partinfo>BBa_R0040 SequenceAndFeatures</partinfo> | ||
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<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
Revision as of 20:04, 17 October 2018
TetR repressible promoter
Sequence for pTet inverting regulator. Promoter is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog, [http://openwetware.org/wiki/ATc aTc].
Usage and Biology
Medium strength promoter. [jb, 5/24/04]
From the reference article:"In contrast to tetracycline, anhydrotetracycline is a particularly useful inducer. It binds Tet R with an ~35-fold higher binding constant and thus allows to operate at very low concentrations. At the same time, its antibiotic activity is ~100-fold lower and concentrations of <50 ng/ml as required for the full induction of P LtetO-1 have no effect on the growth of E.coli."
Improved by Fudan-CHINA in 2018
This year, Fudan-CHINA makes a mutation to Part:BBa_R0040 to improve its combination with the tTA transcriptional factor by computational simulation.Please be free to visit Part:BBa_K2886010.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]