Difference between revisions of "Part:BBa K2569031"
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 19:02, 17 October 2018
PdnaA -NicA2
Expressing this BioBrick in Pseudomonas fluorescence-pf5 cells enables the bacterium to degrade nicotine. NicA2 is a monomer with a size of 53 kDa t, catalyzing the key first step in nicotine catalytic metabolism. Heterologously overexpressing NicA2 in P.fluorescence-pf5 can convert nicotine to pseudo-oxidation can be achieved by resting cells of P.fluorescence-pf5 as a catalyst in a whole-cell reaction.So, with our strong promoper PdnaA enhancing, NicA2 is overexpressed and survived in high concentration of nicotin. And nicotin can get fully degradated.
Nicotine Degradation Pathway
Fig. Nicotin degradation pathway.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1393
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]