Difference between revisions of "Part:BBa K2569031"

Revision as of 18:54, 17 October 2018

PdnaA -NicA2

Expressing this BioBrick in Pseudomonas fluorescence-pf5 cells enables the bacterium to degrade nicotine. NicA2 is a monomer with a size of 53 kDa t, catalyzing the key first step in nicotine catalytic metabolism. Heterologously overexpressing NicA2 in P.fluorescence-pf5 can convert nicotine to pseudo-oxidation can be achieved by resting cells of P.fluorescence-pf5 as a catalyst in a whole-cell reaction.

Nicotine Degradation Pathway

  
  Fig. Nicotine degradation pathway

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1393
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2569031 short</partinfo>
-Expressing this BioBrick in <i>E. coli</i> cells enables the bacterium to degrade nicotine. NicA2 is a monomer with a size of 53 kDa t, catalyzing the key first step in nicotine catalytic metabolism. Heterologously overexpressing NicA2 in <i>E. coli</i> can convert nicotine to pseudo-oxidation can be achieved by resting cells of E. coli as a catalyst in a whole-cell reaction.
+Expressing this BioBrick in <i>Pseudomonas fluorescence</i>-pf5 cells enables the bacterium to degrade nicotine. NicA2 is a monomer with a size of 53 kDa t, catalyzing the key first step in nicotine catalytic metabolism. Heterologously overexpressing NicA2 in <i>P.fluorescence</i>-pf5 can convert nicotine to pseudo-oxidation can be achieved by resting cells of <i>P.fluorescence</i>-pf5 as a catalyst in a whole-cell reaction.
+===Nicotine Degradation Pathway===
 <span class="image fit">
     https://static.igem.org/mediawiki/2018/7/70/T--SKLMT-China--nicotine-pathway.png
-  <p>Fig. Nicotine degradation pathway</p>
+   Fig. Nicotine degradation pathway
 </span>
 ===Usage and Biology===