Difference between revisions of "Part:BBa K2569031"
Line 6: Line 6:
  
 
<span class="image fit">
 
<span class="image fit">
−
<img src="https://static.igem.org/mediawiki/2018/7/70/T--SKLMT-China--nicotine-pathway.png"/>
+
  <img src="https://static.igem.org/mediawiki/2018/7/70/T--SKLMT-China--nicotine-pathway.png"/>
−
<p>Fig. Nicotine degradation pathway</p>
+
  <p>Fig. Nicotine degradation pathway</p>
 
</span>
 
</span>
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 18:50, 17 October 2018


PdnaA -NicA2

Expressing this BioBrick in E. coli cells enables the bacterium to degrade nicotine. NicA2 is a monomer with a size of 53 kDa t, catalyzing the key first step in nicotine catalytic metabolism. Heterologously overexpressing NicA2 in E. coli can convert nicotine to pseudo-oxidation can be achieved by resting cells of E. coli as a catalyst in a whole-cell reaction. 

 <img src="T--SKLMT-China--nicotine-pathway.png"/>

Fig. Nicotine degradation pathway

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1393
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]