Difference between revisions of "Part:BBa K2717022"
 
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In order to improve the expression of mcherry and get a stabilized fluorescence feedback, we add an rbs between emrr Promoter and mCherry.
 
In order to improve the expression of mcherry and get a stabilized fluorescence feedback, we add an rbs between emrr Promoter and mCherry.
  
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<!Plasmid jqy-hairpin is an improved vision of plasmid jqy. Plasmid jqy-hairpin is also a biosensor of Salicylic acid and can also be used as a expression vector of mprA. Because it uses lacO, you need to use IPTG for induction.
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Its sequence includes a circuit of mprA and emrr Binidng Promoter. Mpra is a protein which will bind to emrr Binidng Promoter and cause expressing repression, this repressing function disappears after mprA is binding to Salicylic acid. So the understream gene will express if Salicylic acid exist. The plasmid also has a mCherry sequence as the understream gene of emrr Binding Promoter.
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In order to improve the expression of mcherry and get a stabilized fluorescence feedback, we add an rbs between emrr Promoter and mCherry.-- Add more about the biology of this part here
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===Usage and Biology===
 
===Usage and Biology===
  

Latest revision as of 18:24, 17 October 2018


jqy-hairpin(mprA-Protection coding3-mCherry reverse-scar4-emrr reverse)

Introduction Plasmid jqy-hairpin is an improved vision of plasmid jqy. Plasmid jqy-hairpin is also a biosensor of Salicylic acid and can also be used as a expression vector of mprA. Because it uses lacO, you need to use IPTG for induction. Its sequence includes a circuit of mprA and emrr Binidng Promoter. Mpra is a protein which will bind to emrr Binidng Promoter and cause expressing repression, this repressing function disappears after mprA is binding to Salicylic acid. So the understream gene will express if Salicylic acid exist. The plasmid also has a mCherry sequence as the understream gene of emrr Binding Promoter. In order to improve the expression of mcherry and get a stabilized fluorescence feedback, we add an rbs between emrr Promoter and mCherry.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 451
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 776
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 556