Difference between revisions of "Part:BBa K2717017:Design"
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===Source=== | ===Source=== | ||
| − | + | Sequences were attained from E. coli F12K using PCR. After adding lacO and rbs in the 5’ of mpra while v5 and 6*his tag in the 3’ of it, we fused the altered sequence with emrr Binding Promoter and mCherry. | |
===References=== | ===References=== | ||
Latest revision as of 18:05, 17 October 2018
mprA-protection coding3-mCherry reverse-emrr binding promoter reverse
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 451
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 776
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 556
Design Notes
NOTHING
Source
Sequences were attained from E. coli F12K using PCR. After adding lacO and rbs in the 5’ of mpra while v5 and 6*his tag in the 3’ of it, we fused the altered sequence with emrr Binding Promoter and mCherry.