Difference between revisions of "Part:BBa K2569032"

Latest revision as of 17:42, 17 October 2018

PampC -mRFP

The mRFP behind constitutive promoter PampC, who is the strongest member in our promoter family, leading to enhanced expression of mRFP. lacI is present on the genome of many host cells, which suggests that he normal expression of RFP requires the induction of IPTG and need 18 hours of waiting. We replaced the lac promoter in front of the RFP gene with the strong pf-5 promoter PampC. And hope that RFP, as a visible marker for the naked eye, can be expressed more pronouncedly and faster in in Pseudomonas fluorescens pf-5.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 631
Illegal AgeI site found at 743
1000
COMPATIBLE WITH RFC[1000]

Improvement by replacing induced promoter with constitutive promoter

[http://2018.igem.org/Team:SKLMT-China Team SKLMT-China 2018] Improved the part(BBa_J04450)by replacing induced
promoter with constitutive promoter

lacI is present on the genome of many host cells, which suggests that he normal expression of RFP requires the induction of IPTG
and need 18 hours of waiting. We replaced the lac promoter in front of the RFP gene with the strong pf-5 promoter PampC. And hope that RFP,
as a visible marker for the naked eye, can be expressed more pronouncedly and faster in in Pseudomonas fluorescens pf-5.

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2569032 short</partinfo>
-The mRFP behind constitutive promoter PampC, who is the strongest member in our promoter family, leading to enhanced expression of mRFP. lacI is present on the genome of many host cells, which suggests that he normal expression of RFP requires the induction of IPTG and need 18 hours of waiting. We replaced the lac promoter in front of the RFP gene with the strong pf-5 promoter PampC. And hope that RFP, as a visible marker for the naked eye, can be expressed more pronouncedly and faster in in <latin>Pseudomonas fluorescens </latin>pf-5.
+The mRFP behind constitutive promoter PampC, who is the strongest member in our promoter family, leading to enhanced expression of mRFP. lacI is present on the genome of many host cells, which suggests that he normal expression of RFP requires the induction of IPTG and need 18 hours of waiting. We replaced the lac promoter in front of the RFP gene with the strong pf-5 promoter PampC. And hope that RFP, as a visible marker for the naked eye, can be expressed more pronouncedly and faster in in <i>Pseudomonas fluorescens </i>pf-5.
 <!-- Add more about the biology of this part here
@@ Line 17: / Line 17: @@
 [http://2018.igem.org/Team:SKLMT-China Team SKLMT-China 2018] Improved the part(<partinfo>BBa_J04450</partinfo>)by replacing induced<br/> promoter with constitutive promoter </p>
-  <p>lacI is present on the genome of many host cells, which suggests that he normal expression of RFP requires the induction of IPTG <br/>and need 18 hours of waiting. We replaced the lac promoter in front of the RFP gene with the strong pf-5 promoter PampC. And hope that RFP,<br/> as a visible marker for the naked eye, can be expressed more pronouncedly and faster in in <latin>Pseudomonas fluorescens </latin>pf-5.</p>
+  <p>lacI is present on the genome of many host cells, which suggests that he normal expression of RFP requires the induction of IPTG <br/>and need 18 hours of waiting. We replaced the lac promoter in front of the RFP gene with the strong pf-5 promoter PampC. And hope that RFP,<br/> as a visible marker for the naked eye, can be expressed more pronouncedly and faster in in <i>Pseudomonas fluorescens </i>pf-5.</p>