Difference between revisions of "Part:BBa K2587029"

Revision as of 17:34, 17 October 2018

P_j23102_RBS_ddh_T

Transcription unit with the gene ddh coding for Meso-diaminopimelate D-dehydrogenase. The enzyme catalyzes the reaction from L-2-amino-6-oxopimelate to D,L-2,6-diaminopimelate in the lysine biosynthesis pathway of Corynebacterium glutamicum¹. We use it as a multi-step enzyme for the lysine biosynthesis in Escherichia coli, since the wiltype normally needs five enzymes for the reaction from L-2-amino-6-oxopimelate to D,L-2,6-diaminopimelate.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 422
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 390
Illegal NgoMIV site found at 615
Illegal AgeI site found at 825
1000
COMPATIBLE WITH RFC[1000]

1. Schrumpf, Barbel, et al. "A functionally split pathway for lysine synthesis in Corynebacterium glutamicium." Journal of bacteriology 173.14 (1991): 4510-4516.:
https://jb.asm.org/content/173/14/4510.short

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2587029 short</partinfo>
-Transcription unit with the gene <i>ddh</i> coding for Meso-diaminopimelate D-dehydrogenase. The enzyme catalyses the reaction from L-2-amino-6-oxopimelate to D,L-2,6-diaminopimelate in the lysine biosynthesis pathway of <i>Corynebacterium glutamicum</i><sup>1</sup>. We use it as a multi step enzyme for the lysine biosynthesis in <i>Escherichia coli</i>, since the wiltype normally needs five enzymes for the reaction from L-2-amino-6-oxopimelate to D,L-2,6-diaminopimelate.
+Transcription unit with the gene <i>ddh</i> coding for Meso-diaminopimelate D-dehydrogenase. The enzyme catalyzes the reaction from L-2-amino-6-oxopimelate to D,L-2,6-diaminopimelate in the lysine biosynthesis pathway of <i>Corynebacterium glutamicum</i><sup>1</sup>. We use it as a multi-step enzyme for the lysine biosynthesis in <i>Escherichia coli</i>, since the wiltype normally needs five enzymes for the reaction from L-2-amino-6-oxopimelate to D,L-2,6-diaminopimelate.
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