Difference between revisions of "Part:BBa K2719008:Experience"

Revision as of 20:20, 17 October 2018

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Applications of BBa_K2719008

To confirm the presence of Leptin, it was cloned in pSB1C3 using EcoRI and PstI for the restriction and T4 ligase for the ligation. After that, it was transformed in Escherichia coli DH5a. (Figure 1).

Figure 1. Colonies transformed with leptin coding site.

To prove the presence of the plasmid inside the colonies, two agarose gels were elaborated, one for watch the extraction of the entire plasmid (Figure 2) and the second one for the plasmid with a previously restriction process using NotI, so the last one could confirm the presence of Leptin. (Figure 3)

Figure 2. 0.85% agarose gel with GelRed: MW) 1Kb plus from NEB; 11) Leptin Coding Site (BBa_K2719008) V'; 12) Leptin Coding Site (BBa_K2719008)V

User Reviews

UNIQ2bd4cf181f2b1758-partinfo-00000000-QINU UNIQ2bd4cf181f2b1758-partinfo-00000001-QINU

@@ Line 9: / Line 9: @@
 <p><i>Figure 1.</i> Colonies transformed with leptin coding site. </p>
-<p>To prove the presence of the plasmid inside the colonies, two agarose gels were elaborated, one for watch the entire plasmid and the second one for the plasmid with a previously restriction process using NotI, so the last one could confirm the presence of Leptin. (Figure 4 and 3)</p>
+<p>To prove the presence of the plasmid inside the colonies, two agarose gels were elaborated, one for watch the extraction of the entire plasmid (Figure 2) and the second one for the plasmid with a previously restriction process using NotI, so the last one could confirm the presence of Leptin. (Figure 3)</p>
+[[file:T--TecCEM--LepCS.png|500px]]
+<p><i>Figure 2.</i> 0.85% agarose gel with GelRed: MW) 1Kb plus from NEB; 11) Leptin Coding Site (BBa_K2719008) V'; 12) Leptin Coding Site (BBa_K2719008)V''</p>
 ===User Reviews===