Difference between revisions of "Part:BBa K2719006:Experience"

Revision as of 20:10, 17 October 2018

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Applications of BBa_K2719006

This part was cloned on pSB1C3 using EcoRI and PstI and latter transformed into E.coli DH5a. The colonies obtained can be seen on figure 1.

Figure 1. Colonies transformed with Collagen V-like.

Then the plasmid was extracted and run by duplicated on an agarose gel electrophoresis (Figure 2)

Figure 2. 0.85% agarose gel with GelRed: MW) 1Kb plus from NEB; 3) Collagen V- like (K2719006) V'; 4) Collagen V- like (K2719006) V

Latter it was digested using NotI to probe the presence of the insert, the restriction was also run on a agarose gel electrophoresis.

User Reviews

UNIQ9a60e439230d6ac9-partinfo-00000000-QINU UNIQ9a60e439230d6ac9-partinfo-00000001-QINU

@@ Line 11: / Line 11: @@
-<p>Then the plasmid was extracted and run on an agarose gel electrophoresis, latter it was digested using NotI to probe the presence of the insert, the restriction was also run on a agarose gel electrophoresis.</p>
+<p>Then the plasmid was extracted and run by duplicated on an agarose gel electrophoresis (Figure 2)</p>
+[[file:T--TecCEM--Col5Extraction.png|500px]]
+<p><i>Figure 2.</i> 0.85% agarose gel with GelRed: MW) 1Kb plus from NEB; 3) Collagen V- like (K2719006) V'; 4) Collagen V- like (K2719006) V''</p>
+<p>Latter it was digested using NotI to probe the presence of the insert, the restriction was also run on a agarose gel electrophoresis.</p>
 ===User Reviews===