Difference between revisions of "Part:BBa K2719006:Experience"
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<p>This part was cloned on pSB1C3 using EcoRI and PstI and latter transformed into <i>E.coli</i> DH5a. The colonies obtained can be seen on figure 1.</p> | <p>This part was cloned on pSB1C3 using EcoRI and PstI and latter transformed into <i>E.coli</i> DH5a. The colonies obtained can be seen on figure 1.</p> | ||
| − | [[file:T--TecCEM--Col5Colonies.png]] | + | [[file:T--TecCEM--Col5Colonies.png|500px]] |
<p><i>Figure 1.</i> Colonies transformed with Collagen V-like.</p> | <p><i>Figure 1.</i> Colonies transformed with Collagen V-like.</p> | ||
Revision as of 17:01, 17 October 2018
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how you used this part and how it worked out.
Applications of BBa_K2719006
This part was cloned on pSB1C3 using EcoRI and PstI and latter transformed into E.coli DH5a. The colonies obtained can be seen on figure 1.
Figure 1. Colonies transformed with Collagen V-like.
Then the plasmid was extracted and run on an agarose gel electrophoresis, latter it was digested using NotI to probe the presence of the insert, the restriction was also run on a agarose gel electrophoresis.
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UNIQefbb2dde4d14f6d6-partinfo-00000000-QINU UNIQefbb2dde4d14f6d6-partinfo-00000001-QINU