Difference between revisions of "Part:BBa K2719003:Experience"
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<p>Latter it has extracted and run on an agarose gel electrophoresis (see figure 2).</p> | <p>Latter it has extracted and run on an agarose gel electrophoresis (see figure 2).</p> | ||
| + | [[file:T--TecCEM--GelK2719003.png|500px]] | ||
| + | <p><i>Figure 2.</i> 0.85% agarose gel with GelRed, MW)NEB 1Kb DNA Ladder, 1)Extraction GST Optimized (BBa_K2719003), 2)Extraction GST Optimized (BBa_K2719003) duplicate.</p> | ||
<p>Finally it was treated with NotI and run on an agarose gel electrophoresis to prove the presence of insert (see figure 3).</p> | <p>Finally it was treated with NotI and run on an agarose gel electrophoresis to prove the presence of insert (see figure 3).</p> | ||
Revision as of 21:00, 17 October 2018
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K2719003
This part was cloned on pSB1C3 by restriction with EcoRI and PstI and transformed into E.coli DH5a, obtaining white colonies (see figure 1).
Figure 1. Colonies transformed with optimized GST.
Latter it has extracted and run on an agarose gel electrophoresis (see figure 2).
Figure 2. 0.85% agarose gel with GelRed, MW)NEB 1Kb DNA Ladder, 1)Extraction GST Optimized (BBa_K2719003), 2)Extraction GST Optimized (BBa_K2719003) duplicate.
Finally it was treated with NotI and run on an agarose gel electrophoresis to prove the presence of insert (see figure 3).
User Reviews
UNIQ744496edc2d99cac-partinfo-00000000-QINU UNIQ744496edc2d99cac-partinfo-00000001-QINU