Difference between revisions of "Part:BBa K2533039"
Zyz2018hust (Talk | contribs) |
|||
| Line 6: | Line 6: | ||
<h1>'''Usage and biology'''</h1> | <h1>'''Usage and biology'''</h1> | ||
| − | gapA could transform 3- phosphoglyceraldehyde into 1,3- diphosphoglycerate, mdh could transform malate into pyruvate, pflB helps to transform pyruvate into Acetyl-CoA and fdh helps to transform formate into | + | gapA could transform 3- phosphoglyceraldehyde into 1,3- diphosphoglycerate, mdh could transform malate into pyruvate, pflB helps to transform pyruvate into Acetyl-CoA and fdh helps to transform formate into CO<sub>2</sub>. With the overexpression of gapA-mdh, Shewanella could produce NADH more efficiently, which brings more electricity to be produced. |
<h1>'''Characterization'''</h1> | <h1>'''Characterization'''</h1> | ||
Revision as of 17:03, 17 October 2018
RBS-gapA-RBS-mdh-TT-Ptac-RBS-pflB-RBS-fdh
It encodes glyceraldehyde-3-phosphate dehydrogenase, NAD dependent malate dehydrogenase, pyruvate formate-lyase and formate dehydrogenase cytochrome b.
Usage and biology
gapA could transform 3- phosphoglyceraldehyde into 1,3- diphosphoglycerate, mdh could transform malate into pyruvate, pflB helps to transform pyruvate into Acetyl-CoA and fdh helps to transform formate into CO2. With the overexpression of gapA-mdh, Shewanella could produce NADH more efficiently, which brings more electricity to be produced.
Characterization
This is the whole circuit for NADH production part.
