Difference between revisions of "Part:BBa K2533049"
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<h1>'''Usage and biology'''</h1> | <h1>'''Usage and biology'''</h1> | ||
− | + | It encodes L-lactate permease and D-lactate dehydrogenase, which could produce and transport lactate. mleS could convert malic acid to L-lactate. lldP could transport lactate out of the cell. In this way, Rhodopseudomonas palustris could efficiently transport produced lactate out of the cell, which later will be used by Shewanella. | |
<h1>'''Characterization'''</h1> | <h1>'''Characterization'''</h1> |
Revision as of 16:10, 17 October 2018
RBS-lldP-RBS-ldhA
L-lactate permease & D-lactate dehydrogenase
Usage and biology
It encodes L-lactate permease and D-lactate dehydrogenase, which could produce and transport lactate. mleS could convert malic acid to L-lactate. lldP could transport lactate out of the cell. In this way, Rhodopseudomonas palustris could efficiently transport produced lactate out of the cell, which later will be used by Shewanella.
Characterization
This is one section for lactate utilization part.
DNA Gel Electrophoretic
To make sure that we get the target gene, we did the DNA gel electrophoretic to separate different gene. And here is the result.
Our target genes are 2690bp, and as the marker is DS5000, we could be sure that the bright bands in this picture are our target genes.
Electrogenesis
By comparing the ability of producing electricity, we might find out whether dld could effectively help Shewanella to produce more electricity.
It could be demonstrated that targeted genes could be expressed in the engineered cells. More NADH has been produced by engineered bacteria, which helps to produce more electricty.