Difference between revisions of "Part:BBa K2533049"
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<h2>Electrogenesis</h2> | <h2>Electrogenesis</h2> | ||
By comparing the ability of producing electricity, we might find out whether dld could effectively help Shewanella to produce more electricity. | By comparing the ability of producing electricity, we might find out whether dld could effectively help Shewanella to produce more electricity. | ||
| − | [[File:T--HUST-China--2018-expression of lactate.png |400px|thumb|center|Figure3:shows that our modification is effective. Every gene circuits can help strains produce lactate, and mleS-lldP-ldhA is the most efficient one. Therefore, our construction of gene circuits | + | [[File:T--HUST-China--2018-expression of lactate.png |400px|thumb|center|Figure3:shows that our modification is effective. Every gene circuits can help strains produce lactate, and mleS-lldP-ldhA is the most efficient one. Therefore, our construction of gene circuits achieves the goal to help strains produce lactate.]] |
It could be demonstrated that targeted genes could be expressed in the engineered cells. More NADH has been produced by engineered bacteria, which helps to produce more electricty. | It could be demonstrated that targeted genes could be expressed in the engineered cells. More NADH has been produced by engineered bacteria, which helps to produce more electricty. | ||
Revision as of 15:56, 17 October 2018
RBS-lldP-RBS-ldhA
L-lactate permease & D-lactate dehydrogenase
Usage and biology
dld refers to FAD-dependent D-lactate dehydrogenase which could catalyze D-lactate’s transformation into pyruvate. With the overexpression of dld, Shewanella could utilize D-lactate more efficiently, which brings more electricity being produced.
Characterization
This is one section for lactate utilization part.
DNA Gel Electrophoretic
To make sure that we get the target gene, we did the DNA gel electrophoretic to separate different gene. And here is the result.
Our target genes are 2690bp, and as the marker is DS5000, we could be sure that the bright bands in this picture are our target genes.
Electrogenesis
By comparing the ability of producing electricity, we might find out whether dld could effectively help Shewanella to produce more electricity.
It could be demonstrated that targeted genes could be expressed in the engineered cells. More NADH has been produced by engineered bacteria, which helps to produce more electricty.